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LiCl precipitation offers major advantages over other RNA precipitation methods in that it does not efficiently precipitate DNA, protein or carbohydrate Barlow et al. It also provides a simple rapid method for recovering RNA from in vitro transcription reactions. We have conducted a systematic study of the use of LiCl and find that it is a very effective method for precipitating RNA, especially from in vitro transcription reactions. All of these variables have been explored and are discussed below. We find that LiCl precipitated RNA samples prepared in this way require no further purification for use in hybridization and in vitro translation reactions.

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